亮點(diǎn)：We conducted long-read RNA-sequencing for the CDSI isoforms (37 isoforms in total), whose i-rQTL signals were co-localized with disease GWAS signals and whose unique splice junctions showed significant sQTL signals in LeafCutter analysis (FDR?≤?0.05). The cDNAs were sequenced by MinION (Oxford Nanopore Technologies). We performed conventional long-read RNA-seq using 300?ng of total RNA from LCL and THP-1, then sequenced them using GridION X5 (Oxford Nanopore Technologies).

亮點(diǎn): The Neisseriales order comprises the family Chromobacteriaceae and the family Neisseriaceae and more recently three additional families have been suggested, Aquaspirillaceae, Chitinibacteraceae and Leeiaceae. The family Neisseriaceae includes 12 genera . We selected species from each of these Neisseriaceae genera and used SMRT (PacBio) and Minion (Nanopore) technologies to obtain 21 closed genomes . Genomes obtained in this study were combined with Neisseriaceae draft genomes from the NCBI database to calculate the Average Nucleotide Identity (ANI). This enabled us to identify 75 Neisseriaceae species with genome ANI?>?96%. 

Fig5. Genomic characterization of Empoasca onukii and comparison with other insect genomes. A. Genomic characterization of the sequenced E. onukii. Track

亮點(diǎn): In Asia, the tea green leafhopper (TGL), Empoasca onukii (Hemiptera: Cicadellidae), represents the most devastating pest across tea plantations, causing up to 50% economic loss of tea production annually. We generated a chromosome-level genome assembly of the E. onukii by integrating Illumina short reads, Oxford Nanopore Technologies (ONT) long reads, and high-throughput chromosome conformation capture (Hi-C). This high-quality genome resource enabled us to investigate the genetic basis of chemoreception and detoxification in this insect, key to adapting to new environments.